alexa Simultaneous Estimation of Nebivolol Hydrochloride and Valsartan using RP HPLC.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Kokil SU, Bhatia MS

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Abstract In this study, a rapid, precise, accurate, specific and sensitive ion-paired reverse phase liquid chromatographic method has been developed for the simultaneous estimation of nebivolol hydrochloride and valsartan in their capsule formulation. The chromatographic method was standardized using a HIQ sil C(18) column (250x4.6 mm i.d., 5 mum particle size) with UV detection at 289 nm and flow rate of 1 ml/min. The mobile phase consisting of methanol:water (80:20 v/v) with addition of 0.1 percent 1-hexanesulfonic acid monohydrate sodium salt as an ion-pairing reagent was selected. The method was validated and produced accurate and precise results for estimation of the two drugs.
This article was published in Indian J Pharm Sci and referenced in Journal of Chromatography & Separation Techniques

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