alexa Simultaneous exposure of fish to endosulfan and disulfoton in-vivo: ultrastructural, stereological and biochemical reactions in hepatocytes of male rainbow trout (Oncorhynchusmykiss)
Toxicology

Toxicology

Toxicology: Open Access

Author(s): Helge Arnold, HansJrgen Pluta, Thomas Braunbeck

Abstract Share this page

The response of male rainbow trout (Oncorhynchus mykiss) liver to combined prolonged exposure to 50 ng/l endosulfan (ES) and 0, 1, 5, or 10 μg/l disulfoton (DS) was studied using electron microscopical, stereological and biochemical techniques. Both acute and sublethal toxicities of DS increased exponentially by simultaneous exposure to ES. LC50 was determined at 50 ng/l ES plus 20 μg/l DS. Major sublethal morphological alterations included a 50% increase in cytoplasmic volume due to a 100% increase, dilation and vesiculation of the RER, and a 50% decrease in glycogen deposits. Further reactions were proliferations of mitochondria, myelinated bodies and glycogenosomes, and, at 50 ng/l ES plus 1 μg/l DS only, a transient increase in the absolute volume of peroxisomes and lysosomes. In terms of liver enzyme activities (mU/g liver), biochemical effects comprised induction of ethoxycoumarin- and ethoxyresorufin-O-deethylases, and of glutathione S-transferase, but depression of esterase, acetylcholine esterase, acid phosphatase, aryl sulfatase, glucose 6-phosphate dehydrogenase, malic enzyme and lactate dehydrogenase, as well as increased lipid peroxidation. In terms of specific enzyme activities (mU/mg protein), there was an increase in uricase, aryl sulfatase, glutathione S-transferase, whereas acetylcholine esterase, malic enzyme and esterase were reduced. No consistent changes were seen in succinate dehydrogenase, catalase, glucose 6-phosphate dehydrogenase, lactate dehydrogenase, and NADPH cytochrome P-450 reductase specific activities. Findings indicate a biphasic reaction to combined ES and DS, with a shift from adaptive to degenerative reactions at 5 μg/l DS. Acute toxicity of 50 ng/l ES plus 20 μg/l DS is most likely due to reduced biotransformation capacities by lipid peroxidation-induced membrane damage. Cytochrome P-450 induction and suppression of acetylcholine esterase as typical consequences of ES or DS exposure, respectively, were found as further mechanisms of combined toxicity.

This article was published in Aquatic Toxicology and referenced in Toxicology: Open Access

Relevant Expert PPTs

Relevant Speaker PPTs

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords