Author(s): Watabe S, Yokoyama Y, Nakazawa K, Shinozaki K, Hiraoka R,
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Abstract In this study, three fluoroquinolones, pazufloxacin, ciprofloxacin and levofloxacin, were simultaneously determined in spiked human serum by high-performance liquid chromatography (HPLC) method with fluorescence detection. Chromatography was performed using a C8 column with an isocratic mobile phase consisting of 1\% triethylamine (pH 3.0)/acetonitrile (86/14, v/v). Protein precipitation was conducted using perchloric acid and methanol. The calibration curves for the three fluoroquinolones were linear over concentrations ranging from 0.1 to 20.0 microg/mL. The within-day and between-day coefficients of variation obtained from three fluoroquinolones were less than 7\%, and relative errors ranged from -1.6\% to 9.3\%. Mean recoveries of pazufloxacin, ciprofloxacin, and levofloxacin from spiked human serum were 97\%, 88\%, and 90\%, respectively. The proposed method proved to be simple and reliable for the determination of three fluoroquinolones. Copyright 2010 Elsevier B.V. All rights reserved.
This article was published in J Chromatogr B Analyt Technol Biomed Life Sci
and referenced in Journal of Chromatography & Separation Techniques