alexa Simultaneous quantitation of buspirone and its major metabolite 1-(2-pyrimidinyl)piperazine in human plasma by high-performance liquid chromatography with coulometric detection.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): Odontiadis J, Franklin M

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Abstract Buspirone is a member of the azapirone group of anxiolytic drugs and has one major metabolite, 1-(2-pyrimidinyl)piperazine (1-PP). The analyte, its metabolite and the internal standard were extracted from plasma utilizing solid-phase extraction columns. Chromatography was performed using isocratic reversed-phase high-performance liquid chromatography with coulometric end-point detection. The calibration graph was linear over the range 0-50 ng ml-1 of plasma. The lower limits of quantitation for buspirone and 1-PP were 0.5 and 2 ng ml-1, respectively, when 1 ml of plasma was extracted. The intra-assay relative standard deviations (RSD) over the range of the calibration graph varied from 4 to 12.5\% for buspirone and 1-PP. The inter-assay RSD was 6.9\% for 1-PP and 9.6\% for buspirone. The recovery averaged 96\% for buspirone and 66\% for 1-PP. Plasma profiles of buspirone and 1-PP following oral dosing are presented.
This article was published in J Pharm Biomed Anal and referenced in Pharmaceutica Analytica Acta

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