Author(s): Cordes DB, Miller A, Gamsey S, Singaram B
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Abstract The simultaneous use of several fluorescent reporter dyes in a multicomponent boronic acid-based glucose sensing system is reported. In one application, two dyes with widely different emission wavelengths are used to report changes in glucose concentration. A third glucose-insensitive dye was then added to act as a reference dye and provide for a ratiometric correction to the two reporter dye signals. The inclusion of such a reference dye reduces errors arising from sources such as fluctuations in lamp intensity and sample dilution.
This article was published in Anal Bioanal Chem
and referenced in Journal of Glycomics & Lipidomics