alexa Single living cell detection of telomerase over-expression for cancer detection by an optical fiber nanobiosensor.


Biosensors Journal

Author(s): Zheng XT, Li CM

Abstract Share this page

Abstract An optical fiber nanobiosensor was constructed to successfully detect a general cancer biomarker, telomerase at single cell level with its nanoscale tip. The nanotip immobilized with a specific antibody was inserted into a MCF-7 breast cancer cell nucleus to capture telomerases directly, after which an in vitro enzymatic sandwich immunoassay was performed to achieve sensitive single living cell detection. The nanotip inserted into MCF-7 cell nucleus provides significantly higher average (F-F(0))/F(0) ratio than that of human mesenchymal stem cell (hMSC) nucleus, demonstrating the successful detection of the telomerase over-expression in cancer cells as compared to normal cells. The detection in the cytoplasm shows much smaller average ratio than that in the nucleus of MCF-7 cells while clearly verifies the nuclear localization of telomerase. The successful detection of telomerase over-expression in a single living cell for the first time may provide a potential method for cancer detection, and also demonstrate a universal approach that can be used to detect other low expression proteins in a single living cell. Copyright 2009 Elsevier B.V. All rights reserved. This article was published in Biosens Bioelectron and referenced in Biosensors Journal

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version