alexa Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Next Generation Sequencing & Applications

Author(s): Smallwood SA, Lee HJ, Angermueller C, Krueger F, Saadeh H,

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Abstract We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4\% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
This article was published in Nat Methods and referenced in Journal of Next Generation Sequencing & Applications

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