Author(s): Chomczynski P, Sacchi N
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Abstract A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
This article was published in Anal Biochem
and referenced in Biochemistry & Physiology: Open Access