alexa Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.


Advancements in Genetic Engineering

Author(s): Winans SC, Elledge SJ, Krueger JH, Walker GC

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Abstract A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be crossed into the gene's original replicon by linearizing the cloned DNA and transforming a recB recC sbcB mutant. A number of applications of this method are described with genes of either chromosomal or plasmid origin.
This article was published in J Bacteriol and referenced in Advancements in Genetic Engineering

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