alexa Sites of metal deposition in the cell wall of Bacillus subtilis.


Journal of Microbial & Biochemical Technology

Author(s): Beveridge TJ, Murray RG, Beveridge TJ, Murray RG

Abstract Share this page

Abstract Amine and carboxyl groups of the cell wall of Bacillus subtilis were chemically modified individually to neutralize their electrochemical charge for determination of their contribution to the metal uptake process. Mild alkali treatment removed ca. 94\% of the constituent teichoic acid (expressed as inorganic phosphorus) and allowed estimation of metal interaction with phosphodiester bonds. Chemical modifications of amine functions did not reduce the metal uptake values as compared to native walls, whereas extraction of teichoic acid caused a stoichiometric reduction in levels. In contrast, alteration of carboxyl groups severely limited metal deposition of most of the metals tested. X-ray diffraction and electron microscopy suggested, in this case, that the form and structure of the metal deposit could be different from that found in native walls. The observations suggest that carboxyl groups provide the major site of metal deposition in the B. subtilis wall.
This article was published in J Bacteriol and referenced in Journal of Microbial & Biochemical Technology

Relevant Expert PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version