alexa Size separation of circulatory DNA in maternal plasma permits ready detection of fetal DNA polymorphisms.


Angiology: Open Access

Author(s): Li Y, Zimmermann B, Rusterholz C, Kang A, Holzgreve W,

Abstract Share this page

Abstract BACKGROUND: Analysis of fetal DNA in maternal plasma has recently been introduced as a new method for noninvasive prenatal diagnosis, particularly for the analysis of fetal genetic traits, which are absent from the maternal genome, e.g., RHD or Y-chromosome-specific sequences. To date, the analysis of other fetal genetic traits has been more problematic because of the overwhelming presence of maternal DNA sequences in the circulation. We examined whether different biochemical properties can be discerned between fetal and maternal circulatory DNA. METHODS: Plasma DNA was examined by agarose gel electrophoresis. The fractions of fetal and maternal DNA in size-fractionated fragments were assayed by real-time PCR. The determination of paternally and maternally inherited fetal genetic traits was examined by use of highly polymorphic chromosome-21-specific microsatellite markers. RESULTS: Size fractionation of circulatory DNA indicated that the major portion of cell-free fetal DNA had an approximate molecular size of <0.3 kb, whereas maternally derived sequences were, on average, considerably larger than 1 kb. Analysis of size-fractionated DNA (
  • DOI: 10.1373/clinchem.2003.029835
  • This article was published in Clin Chem and referenced in Angiology: Open Access

    Relevant Expert PPTs

    Relevant Speaker PPTs

    Recommended Conferences

    • International Conference on Angiology
      Oct 16-17, 2017, Budapest, Hungary
    • 21st International Conference on Clinical & Experimental Cardiology
      October 16-18, 2017 Chicago, USA

    Relevant Topics

    Peer Reviewed Journals
    Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
    International Conferences 2017-18
    Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

    Contact Us

    © 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version