Author(s): Luchini A, Geho DH, Bishop B, Tran D, Xia C,
Abstract Share this page
Abstract Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.
This article was published in Nano Lett
and referenced in Journal of Sports Medicine & Doping Studies