alexa Specific detection of Campylobacter concisus by PCR amplification of 23S rDNA areas.


Clinical Microbiology: Open Access

Author(s): Bastyns K, Chapelle S, Vandamme P, Goossens H, De Wachter R

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Abstract The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. concisus, they are too laborious to be performed in routine settings. Hence a simple Campylobacter concisus-specific PCR assay was developed, based on a target sequence which comprises the most variable areas of 23S rDNA. The PCR assay was successfully evaluated on a broad selection of C. concisus strains and phylogenetically related bacteria.
This article was published in Mol Cell Probes and referenced in Clinical Microbiology: Open Access

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