Author(s): Fukuda K, Vishinuvardhan D, Sekiya S, Kakiuchi N, Shimotohno K,
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Abstract In order to isolate RNA aptamers that bind specifically to NS3 protease domain (delta NS3) of hepatitis C virus, we carried out in vitro selection procedure using RNA pool that had 30 N random core region. After repeating nine cycles of selections and amplifications, a pool of RNAs that bind specifically to the delta NS3 were selected. A comparative analysis of 45 clones that were isolated from 9th cycle revealed three main classes that contain the conserved loop sequences GANUGGGAC. Moreover, the predominant class of aptamer (class I and III) appear to inhibit the protease activity efficiently.
This article was published in Nucleic Acids Symp Ser
and referenced in Biochemistry & Physiology: Open Access