Author(s): Vilchez JL, Blanc R, Avidad R, Navaln A
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Abstract A spectrofluorimetric method for the determination of paracetamol is presented, based on the oxidation of the analyte to give the fluorophore 2,2'-dihydroxy-5,5'-diacetyldiaminebiphenyl. Sodium hypochlorite was used as an oxidizing reagent and the optimum pH was found to be 10.0 (sodium carbonate-boric acid buffer solution). The linear concentration range of application was 0.1-100.0 micrograms ml-1 of paracetamol, the detection limit 0.01 micrograms ml-1 and the relative standard deviation 1.2\%. The method has been satisfactorily applied to the determination of paracetamol in pharmaceutical formulations and biological fluids.
This article was published in J Pharm Biomed Anal
and referenced in Pharmaceutica Analytica Acta