alexa Spectrophotometric and spectrofluorimetric determination of etodolac and aceclofenac.
Pharmaceutical Sciences

Pharmaceutical Sciences

Pharmaceutica Analytica Acta

Author(s): El Kousy NM

Abstract Share this page

Abstract Two simple, sensitive and reproducible spectrophotometric and spectrofluorimetric methods were adopted for the analysis of the anti-inflammatory drugs, etodolac and aceclofenac. The first method is based on the formation of coloured complexes between the drugs and p-dimethylaminobenzaldehyde reagent (PDAB) in the presence of sulfuric acid and ferric chloride. Measurement of the absorbances was carried out at 591.5 and 545.5 nm for etodolac and aceclofenac, respectively. Regression analysis of Beer's plots showed good correlation in the concentration ranges 10-80 and 8-55 microg ml(-1), respectively. The second was the spectrofluorimetric method in which samples of etodolac in ethanol showed native fluorescence at a lambda = 345 nm when excitation was at 235 nm and samples of aceclofenac in the phosphate buffer pH 8 showed native fluorescence at lambda = 355 nm when excitation was at 250 nm. The calibration graph was rectilinear from 96 to 640 ng ml(-1) for etodolac and from 2 to 8 microg ml(-1) for aceclofenac. The proposed methods are applied successfully for the determination of the two drugs in bulk powder with a mean accuracy of 100.48+/-0.85 and 100.03+/-0.38 in the PDAB method and of 100.61+/-0.79 and 99.88+/-0.45 in the spectrofluorimetric method. Applicability of the proposed methods was examined by analysing dosage forms of the investigated drugs. Recoveries were 98.77-101.46 and 98.65-102.10\% for the two methods, respectively and RSD values were 0.6-0.7 and 0.35-1.06\% respectively.
This article was published in J Pharm Biomed Anal and referenced in Pharmaceutica Analytica Acta

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords