alexa Spectrophotometric simultaneous determination of nitrite, nitrate and ammonium in soils by flow injection analysis.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Proteomics & Bioinformatics

Author(s): Lpez Pasquali CE, Fernndez Hernando P, Durand Alegra JS

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Abstract A new rapid flow injection procedure for the simultaneous determination of nitrate, nitrite and ammonium in single flow injection analysis system is proposed. The procedure combines on-line reduction of nitrate to nitrite and oxidation of ammonium to nitrite with spectrophotometric detection of nitrite by using the Griess-Ilosvay reaction. The formed azo dye was measured at 543 nm. The influence of reagent concentration and manifold parameters were studied. Nitrite, nitrate and ammonium can be determined within the range of 0.02-1.60 microgmL(-1), 0.02-1.60 microgmL(-1) and 0.05-1.40 microgmL(-1), respectively. R.S.D. values (n=10) were 2.66; 1.41 and 3.58 for nitrate, nitrite and ammonium, respectively. This procedure allows the determination and speciation of inorganic nitrogen species in soils with a single injection in a simple way, and high sampling rate (18 h(-1)). Detection limits of 0.013, 0.046 and 0.047 microgmL(-1)were achieved for nitrate, nitrite and ammonium, respectively. In comparison with others methods, the proposed one is more simple, it uses as single chromogenic reagent less injection volume (250 mL in stead of 350 mL) and it has a higher sampling rate. This article was published in Anal Chim Acta and referenced in Journal of Proteomics & Bioinformatics

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