alexa Spermine, another specific allosteric activator of calcium uptake in rat liver mitochondria.


Biochemistry & Analytical Biochemistry

Author(s): Krner H

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Abstract Calcium uptake in rat liver mitochondria is accelerated by spermine. At a concentration of 2 microM Ca2+ and 1 mM Mg2+ a maximal, 10-fold activation by 1.2 mM spermidine was obtained; a half-maximal activation was attained with 0.2 mM spermine. Spermidine was far less effective than spermine whereas putrescine was ineffective. The acceleration of Ca uptake at low, physiological Ca2+ concentrations is related to the altered kinetics of the Ca uniporter. Corresponding to the alteration by high Ca2+ concentrations previously described, the kinetics changed from sigmoidal in the absence to nearly hyperbolic in the presence of spermine. Mg2+ behaves as an allosteric inhibitor. This phenomenon of the allosteric activation of Ca uptake could not be observed in heart mitochondria.
This article was published in Arch Biochem Biophys and referenced in Biochemistry & Analytical Biochemistry

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