Author(s): Suresh P, Wanchu A, Bhatnagar A, Sachdeva RK, Sharma M, Suresh P, Wanchu A, Bhatnagar A, Sachdeva RK, Sharma M
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Abstract Exposure to human immunodeficiency virus (HIV)-1 does not inevitably result in infection and resistance to HIV-1 infection is observed in different categories of at-risk individuals. In this study, the role of beta-chemokines and alpha-chemokine in providing resistance to HIV-1 infection was evaluated in a group of 25 HIV-exposed but uninfected (EU) partners of HIV-1-infected individuals. We studied the levels of regulated on activation, normal T expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta , and stromal cell-derived factor (SDF)-1alpha in culture supernatants of peripheral blood mononuclear cells (PBMCs) after stimulation with HIV gag p24 antigen and phytohemagglutinin (PHA). Higher gag-specific beta-chemokine responses were seen in EU individuals and HIV-positive controls when compared with healthy controls (HC). No significant difference was observed in PHA-specific beta-chemokine production between these three groups. Moreover, a spontaneous production of all the three beta-chemokines by unstimulated PBMCs was observed in EU individuals and HIV-positive controls. No significant difference was observed in alpha-chemokine (SDF-1) levels between the three groups after p24 and PHA stimulation. We conclude that in our cohort of EU individuals, beta-chemokines-mediated resistance against HIV might be present. Since beta-chemokines are produced mainly by activated T cells, our results suggest that enhanced chemokine production might be due to exposure to HIV in these individuals.
This article was published in AIDS Res Hum Retroviruses
and referenced in Journal of AIDS & Clinical Research