alexa Square wave voltammetric sensing of uric acid using the self-assembly of mercaptobenzimidazole.
Chemical Engineering

Chemical Engineering

Journal of Analytical & Bioanalytical Techniques

Author(s): Raj CR, Kitamura F, Ohsaka T

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Abstract The self-assembled monolayer of a heterocyclic thiol, mercaptobenzimidazole (MBI) on gold (Au) electrode is successfully utilized for the voltammetric sensing of uric acid (UA). The self-assembly of MBI separates the voltammetric signal of UA from the interfering ascorbate (AA). Selective detection of UA in the presence of a large excess of AA or the simultaneous detection of UA and AA is achieved at the MBI monolayer-modified electrode. This electrode can detect as low as 1 microM of UA in the presence of 100-fold excess of AA with excellent reproducibility. The practical utility of the electrode is demonstrated by measuring the concentration of UA in human serum.
This article was published in Analyst and referenced in Journal of Analytical & Bioanalytical Techniques

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