alexa Stability of haloalkaliphilic Geomicrobium sp. protease modulated by salt.
Biochemistry

Biochemistry

Enzyme Engineering

Author(s): Karan R, Khare SK

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Abstract A novel protease from the halophilic bacterium Geomicrobium sp. EMB2 (MTCC 10310) is described. The activity of the protease was modulated by salt, and it exhibited remarkable stability in organic solvents, at alkaline pH, and in other denaturing conditions. The structural changes under various denaturing conditions were analyzed by measurements of intrinsic fluorescence and circular dichroism spectroscopy. Circular dichroism showed that the secondary structure of the protease was predominantly α-helical but unfolded in salt-free medium. The structure is regained by inclusion of NaCl in the range of 2-5\%. The presence of NaCl exerted a protective effect against thermal, organic solvent, and guanidine hydrochloride denaturation by preventing unfolding. This article was published in Biochemistry (Mosc) and referenced in Enzyme Engineering

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