Author(s): Li T, Zhang J
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Abstract Retroviral vectors are used widely to deliver heterologous genes into cells. In order to express three genes from a single RNA molecule, a retroviral vector that contains two divergent internal ribosome entry site (IRES) sequences has been constructed successfully. To eliminate the high frequency of recombination within a mulicistronic retrovirus vector, a 9-nt segment of a cellular mRNA IRES and a picornaviral IRES were used, since these two IRES sequences have minimal sequence homology. After a single round of replication, most cells infected with this vector stably expressed the three genes while approximately 40\% of cells infected with another tricistronic retroviral vector that contains two copies of an identical IRES sequence lost expression of the gene located between these two sequences.
This article was published in J Virol Methods
and referenced in Cloning & Transgenesis