Author(s): Rodrguez Couto S, Sanromn MA, Hofer D, Gbitz GM
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Abstract Alginate beads, polyurethane foam, nylon sponge and stainless steel sponge were tested as carrier materials for the white-rot fungus Trametes hirsuta for laccase production under submerged fermentation conditions. Stainless steel sponge was the best carrier material leading to the highest laccase activities of up to 800 U/l after 8 days of cultivation. These values are higher than those reported to date operating with inert supports and without inducer addition. In a 1-l bioreactor containing T. hirsuta immobilised on stainless steel sponge laccase activities of about 2200 U/l were obtained when the culture medium was supplemented with 1 mM copper sulphate. There were no operational problems with this system during culturing time. The textile dye Indigo Carmine was almost totally degraded in 3 days by T. hirsuta grown in this bioreactor, while Lanaset Marine was degraded in two successive batches, reaching in the first batch a decolourization percentage of about 82\% in 15 h and in the second one by 71\% in 28 h. Results obtained after inhibition of growth of T. hirsuta by antibiotics indicated that dye decolourization could not exclusively be attributed to laccase activity.
This article was published in Bioresour Technol
and referenced in Journal of Aquaculture Research & Development