alexa Standardization of hemoglobinometry II. The hemiglobincyanide method


Journal of Vaccines & Vaccination

Author(s): EJ van Kampen, WG Zijlstra

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An easy and reliable (spectro-)photometric procedure is described for the determination of hemoglobin as hemiglobincyanide. The time needed for a determination has been shortened from 20 to 4 min by shifting the ph of the diluent from 8.6 to 7.2. This has been accomplished by substituting (140 mg/l) KH2PO4 for the NaHCO3 in the diluent conventionally used in the HiCN method. Turbidity through precipitation of plasma proteins is prevented by the addition of a small amount of a detergent (Sterox SE) which also promotes rapid lysis of the red cells.

This article was published in Clinica Chimica Acta and referenced in Journal of Vaccines & Vaccination

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