alexa Static biofilm cultures of Gram-positive pathogens grown in a microtiter format used for anti-biofilm drug discovery.


Journal of Microbial & Biochemical Technology

Author(s): Kwasny SM, Opperman TJ

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Abstract An in vitro assay is presented for culturing staphylococcal biofilms and biofilms of nonmotile Gram-positive bacteria under static conditions in microtiter assay plates, and for the quantification of biofilm growth, using a simple staining procedure that measures amounts of bacterial cells and extracellular matrix. This basic assay can be adapted readily to study several aspects of biofilm formation, for high-throughput screening to identify small molecule inhibitors of biofilm formation or biofilm-defective mutants, and for quantifying the anti-biofilm activity of biofilm inhibitors. © 2010 by John Wiley & Sons, Inc.
This article was published in Curr Protoc Pharmacol and referenced in Journal of Microbial & Biochemical Technology

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