Author(s): Taran M, Amirkhani H
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Abstract We investigated optimization of poly(3-hydroxybutyrate) (PHB) production by Haloarcula sp. IRU1 in a batch culture system. Various batch experiments were carried out at different carbon source concentrations [glucose, at 1-8\% (w/v)], phosphorus source concentrations [K(2)HPO(4), KH(2)PO(4), Na(3)PO(4) and Na(2)HPO(4) at 0.001-0.016\% (w/v)], nitrogen source concentrations [NH(4)Cl, yeast extract, peptone and tryptone at 0.1-0.8\% (w/v)] and temperatures [37-55°C]. By these experiments, optimum production conditions were determined using the Taguchi method. The results showed the highest PHB production by Haloarcula sp. IRU1 (63.0\% of cell dry weight) in the presence of 2g/l glucose, 0.2g/l NH(4)Cl, 0.004 g/l KH(2)PO(4) and temperature (42°C). In overall, the results of this study showed that Haloarcula sp. IRU1 has a high potential for synthesis of PHB from glucose. Copyright © 2010 Elsevier B.V. All rights reserved.
This article was published in Int J Biol Macromol
and referenced in Journal of Petroleum & Environmental Biotechnology