alexa Structures of the complex glycans found on the beta-subunit of (Na,K)-ATPase.
Bioinformatics & Systems Biology

Bioinformatics & Systems Biology

Journal of Glycomics & Lipidomics

Author(s): Treuheit MJ, Costello CE, Kirley TL

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Abstract (Na,K)-ATPase is an integral membrane protein responsible for maintaining the Na+ and K+ ion concentration gradients across the plasma membranes of cells. All active (Na,K)-ATPase preparations consist of two subunits, designated alpha and beta. The alpha-subunit is the catalytic subunit and contains the cardiac glycoside binding site. In contrast, the physiological function of the beta-subunit remains unclear although it appears to be involved in the processes of folding, membrane insertion, and stabilization of the alpha-subunit. Previous work has determined the amino acid sequence and disulfide bond arrangements for the beta-subunit from both lamb and dog kidney. In this report, we describe the isolation and structural characterization of the glycan moieties of the beta-subunit from both lamb and dog kidney (Na,K)-ATPase. The three glycosylation sites of these beta-subunits were fractionated using reverse phase chromatography after cleavage of the polypeptide chain with trypsin and thermolysin. Glycopeptides derived from each glycosylation site were analyzed by matrix-assisted laser desorption ionization mass spectrometry. The mass spectrometry results indicated that the predominant glycoforms at the three glycosylation sites of these beta-subunits were a combination of the tetraantennary glycan form and the unusual glycan form of tetraantennary with a limited number of repeating N-acetyllactosamine units. These results further define the covalent structure for the beta-subunit from both lamb and dog kidney (Na,K)-ATPase and suggest that the beta-subunit may be derived from an adhesion molecule.
This article was published in J Biol Chem and referenced in Journal of Glycomics & Lipidomics

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