Author(s): Zhou H, Li S, Huang G, Liu N
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Abstract OBJECTIVE: To probe inhibition effect of zinc-saturated (Zn2+ -BLF), iron-saturated (Fe2+ -BLF) and manganese-saturated (Mn2+ -BLF) bovine lactoferrin on hepatitis B virus DNA (HBV-DNA) in vitro. METHODS: Nature HBV was used to infect HepG2 cell, HBV-DNA was measured by fluorescence polymerase chain reaction to detect the effect of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF, the MTT test was used to examine cytotoxic effect of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF. RESULTS: The maximum nontoxic dose (TD0) in HepG2 cell of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF were 1.5,3.0 and 1.5 g/L respective. After HepG2 cell were infected with HBV, every Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF test group had effect on inhibiting HBV-DNA to some extent. Compared the inhibition effect of concentration of 1.5 g/ L test group in each term, the inhibition result of Zn2 -BLF was 5.746 +/- 0.114, the inhibition result of Fe2+ -BLF was 6. 446 +/- 0.103, and the inhibition result of Mn2+ -BLF was 5.999 +/- 0.725. CONCLUSION: After HepG2 cell was infected with HBV, HBV-DNA could be inhibited by Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF. Zn2+ -BLF has higher inhibition effect than Mn2+ -BLF, and Fe2+ -BLF has the lowest inhibition effect, but the possible mechanism need to be lucubrated.
This article was published in Wei Sheng Yan Jiu
and referenced in Journal of Clinical & Cellular Immunology