alexa Subcellular localization of an extracellular serine protease in Leishmania (Leishmania) amazonensis.


Journal of Vaccines & Vaccination

Author(s): SilvaLopez RE, MorgadoDaz JA, Alves CR, CrteReal S, GiovanniDeSimone S

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Abstract Extracellular proteolytic activity was detected in a Leishmania ( L.) amazonensis culture supernatant and a 56-kDa protein was purified using (NH4)2SO4 precipitation followed by affinity chromatography on aprotinin-agarose. A rabbit serum obtained against the 56-kDa extracellular serine protease was used in order to analyze its location in L. ( L.) amazonensis parasites. Immunocytochemistry studies revealed that the enzyme is mainly found in the flagellar pocket and cytoplasmic vesicles of promastigote forms, whereas in amastigotes, it is located in electron-dense structures resembling megasomes. These results indicate that the 56-kDa serine protease is released into the extracellular environment through the flagellar pocket; and its intracellular location suggests either a correlated enzymatic activity or intracellular trafficking. Copyright 2004 Springer-Verlag This article was published in Parasitol Res and referenced in Journal of Vaccines & Vaccination

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