Author(s): Geiger T, Cox J, Ostasiewicz P, Wisniewski JR, Mann M
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Abstract We describe a method to accurately quantify human tumor proteomes by combining a mixture of five stable-isotope labeling by amino acids in cell culture (SILAC)-labeled cell lines with human carcinoma tissue. This generated hundreds of thousands of isotopically labeled peptides in appropriate amounts to serve as internal standards for mass spectrometry-based analysis. By decoupling the labeling from the measurement, this super-SILAC method broadens the scope of SILAC-based proteomics.
This article was published in Nat Methods
and referenced in Journal of Clinical & Cellular Immunology