Author(s): Lebrecht A, Boehm D, Schmidt M, Koelbl H, Grus FH
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Abstract BACKGROUND: New biomarkers are needed to improve the early detection of breast cancer. This study describes the use of surface-enhanced laser desorption/ionisation time-of-flight mass spectroscopy (SELDI-TOF-MS) in serum and tear fluid. MATERIALS AND METHODS: Blood and tear fluid of 10 women with breast cancer and 10 healthy age-matched women were screened for potential biomarkers. Blood samples and tear fluid were drawn prior to surgery. SELDI-TOF-MS were used for protein profiling with three different active surfaces of the protein chips. The data were analyzed by multivariate statistical techniques and artificial neural networks. RESULTS: Complex protein and peptide patterns were found on all three surfaces. We identified the main proteins in tear fluid. Statistically significant differences in the protein pattern (p<0.001) were found between breast cancer patients and healthy controls. The diagnostic pattern differentiated cancer patients from controls with a specificity and sensitivity of approximately 90\% in serum and tear fluid. CONCLUSION: Protein chip technology facilitates the discovery of new and better biomarkers in breast cancer. It is a promising approach to analyse a large number of patients with high sensitivity and specificity. Analysing tear fluid could show some advantages.
This article was published in Cancer Genomics Proteomics
and referenced in Journal of Cytology & Histology