Author(s): Montel Mendoza G, Pasteris SE, Otero MC, Fatima NaderMacas ME
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Abstract AIM: To evaluate the effect of freeze-drying and storage conditions on the viability and beneficial properties of lactic acid bacteria (LAB) for raniculture. METHODS AND RESULTS: Lactococcus lactis CRL 1584, L. lactis CRL 1827, Lactococcus garvieae CRL 1828 and Lactobacillus plantarum CRL 1606 viability under different conditions was studied. 10\% lactose and 5\% skim milk + 5\% lactose were excellent lyoprotectants, but 5\% skim milk + 5\% lactose and whey protein concentrated (WPC) or WPC + sugars were the lower cost lyoprotective options. The effect of temperature depended on both lyoprotectants and storage time. Thus, for Lactococcus, skim milk, skim milk + sucrose and WPC + sucrose were selected for lyophilization and storage at 4°C and skim milk + lactose for 25°C. For Lact. plantarum CRL 1606, the best lyoprotectants for lyophilization and storage at 4°C were milk + sugars and WPS + sucrose and, at 25°C, skim milk + sucrose. CONCLUSIONS: Lactic acid bacteria viability after freeze-drying was strain-specific and depended on the lyoprotectant used. Highest viability was obtained when stored at 4°C, and the beneficial properties remained stable for 18 months independently of storage temperature. SIGNIFICANCE AND IMPACT OF THE STUDY: The studies reported for the first time in this work are of primary interest to obtain dried bacteria to be included in beneficial products for raniculture. © 2013 The Society for Applied Microbiology.
This article was published in J Appl Microbiol
and referenced in Journal of Bioprocessing & Biotechniques