alexa Synthesis of functional protein in liposome.
Chemical Engineering

Chemical Engineering

Journal of Chemical Engineering & Process Technology

Author(s): Yu W, Sato K, Wakabayashi M, Nakaishi T, KoMitamura EP, , Yu W, Sato K, Wakabayashi M, Nakaishi T, KoMitamura EP, , Yu W, Sato K, Wakabayashi M, Nakaishi T, KoMitamura EP, , Yu W, Sato K, Wakabayashi M, Nakaishi T, KoMitamura EP,

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Abstract The liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture. The synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from the liposome on flow cytometry analysis and fluorescence microscopy. The protein synthesized in the liposome is hence functional.
This article was published in J Biosci Bioeng and referenced in Journal of Chemical Engineering & Process Technology

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