Author(s): Wang H, Hu YC, Markoulaki S, Welstead GG, Cheng AW,
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Abstract The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes--Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.
This article was published in Nat Biotechnol
and referenced in Cloning & Transgenesis