Author(s): Cho SW, Kim S, Kim JM, Kim JS
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Abstract We employ the CRISPR-Cas system of Streptococcus pyogenes as programmable RNA-guided endonucleases (RGENs) to cleave DNA in a targeted manner for genome editing in human cells. We show that complexes of the Cas9 protein and artificial chimeric RNAs efficiently cleave two genomic sites and induce indels with frequencies of up to 33\%.
This article was published in Nat Biotechnol
and referenced in Gene Technology