alexa Targeting Nitric Oxide Mediated U pregulatio n of M embrane - bound Glucose R egulated - protein 78 by S ubtractive Single Chain V ariable Fragment (scFv) Phage D isplay
Genetics

Genetics

Single Cell Biology

Author(s): Lizhi Liu, Weiming Xu

Abstract Share this page

Nitric oxide (NO) is a pleiotropic signali ng molecule, and may be a contributing factor to the hypoxia encountered in a variety of solid tumors. Previously we reported that increased NOS expression resulted in a concomitant increase in expression of the glucose - regulated protein 78 (GRP78) in huma n embryonic kidney 293 cells. In the present study, we found that GRP78 was expressed on the cell membrane after nitric oxide induction. In order to isolate membrane - bound GRP78, we have designed a novel scFv library screening method, based on alternative screening with recombinant protein and cell surface - expressed antigen. After first routine screen using GRP78 protein, we used NO - induced cell surface GRP78 as a bait to subtract the none - membrane binding phages. We have successful isolated a panel of scFv specific for GRP78. Treatment of cells with one of the scFv (scFv - GRP78 - H19) attenuated the GRP78 - mediated protection against thapsigargin, a selective ER Ca 2+ - ATPase inhibitor. Furthermore, it synergized with ionomycin (IM) to promote apoptosis in prost ate cancer cells and inhibited the glucose - deprivation - mediated etoposide resistance in human colon and prostate cancer. The work presented here has highlighted the possibility that anti - GRP78 antibodies have the potential to be used therapeutically for th e treatment of cancer.

  • To read the full article Visit
  • Open Access
This article was published in American Journal of Biomedical Sciences and referenced in Single Cell Biology

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords