Author(s): Liu Q, Scheller KK, Schaefer DM
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Abstract A simplified method for the determination of alpha-tocopherol concentration in beef muscle was developed and evaluated. The method consists of a saponification step applied to 1-g samples of intact, fresh muscle, followed by a single isooctane extraction of the saponified samples. alpha-Tocopherol in the extract was separated by normal phase chromatography and quantified by fluorescence detection. A single extraction with the simplified method accounted for 95\% of the total muscle alpha-tocopherol concentration obtained by two extractions with the Arnold et al. (J. Food Sci. 58:28, 1993) method. Recovery of added alpha-tocopherol standard after two extractions of a saponified muscle sample was 91\% for the simplified method, which was not different (alpha = .78) from recovery using the Arnold method, and the efficiency of the single extraction in the simplified method was 89\%. The coefficients of variation for the simplified and Arnold methods were both 3.1\%. This method should permit the duplicate analysis of 100 fresh muscle samples within 24 h.
This article was published in J Anim Sci
and referenced in Journal of Analytical & Bioanalytical Techniques