alexa Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens.
Microbiology

Microbiology

Journal of Microbial & Biochemical Technology

Author(s): RodrguezMoya J, Argandoa M, IglesiasGuerra F, Nieto JJ, Vargas C

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Abstract Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.
This article was published in Appl Environ Microbiol and referenced in Journal of Microbial & Biochemical Technology

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