alexa Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Blagoev B, Ong SE, Kratchmarova I, Mann M

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Abstract To study the global dynamics of phosphotyrosine-based signaling events in early growth factor stimulation, we developed a mass spectrometric method that converts temporal changes to differences in peptide isotopic abundance. The proteomes of three cell populations were metabolically encoded with different stable isotopic forms of arginine. Each population was stimulated by epidermal growth factor for a different length of time, and tyrosine-phosphorylated proteins and closely associated binders were affinity purified. Arginine-containing peptides occurred in three forms, which were quantified; we then combined two experiments to generate five-point dynamic profiles. We identified 81 signaling proteins, including virtually all known epidermal growth factor receptor substrates, 31 novel effectors and the time course of their activation upon epidermal growth factor stimulation. Global activation profiles provide an informative perspective on cell signaling and will be crucial to modeling signaling networks in a systems biology approach. This article was published in Nat Biotechnol and referenced in Journal of Chromatography & Separation Techniques

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