alexa Temporal and spatial gene expression patterns after experimental stroke in a rat model and characterization of PC4, a potential regulator of transcription.


Biochemistry & Analytical Biochemistry

Author(s): Roth A, Gill R, Certa U

Abstract Share this page

Abstract We have used the middle cerebral artery occlusion model in the rat in combination with microarray transcript imaging to study changes in gene activity after ischemic stroke. We analyze transcriptional changes in three regions of the affected, ipsilateral brain sphere using contralateral tissues from the same animal as a control over several time points in 180 individual RNA samples. After 1 h transcription factors and signaling molecules are expressed in all tissues followed by the induction of tissue repair-related genes in the cortices which undergo regeneration. Some of these genes are turned on by PC4, which is upregulated in tissues surrounding the infarct core. Interestingly, PC4 is a nerve growth factor (NGF)-inducible gene and has been associated in earlier studies with neuronal growth processes. The expression mode of PC4, the cellular localization of the gene product, and the functional properties of downstream genes induced in vivo and in vitro using transgenic cell lines suggest that PC4 is a regulator of transcription involved in tissue regeneration after ischemic stroke. The novel experimental strategy applied here is suited to provide insight into the molecular mechanisms underlying stroke and tissue regeneration and may enable the discovery of preventive medicines.
This article was published in Mol Cell Neurosci and referenced in Biochemistry & Analytical Biochemistry

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version