alexa The cattle interleukin-13 gene: genomic organization, chromosomal location, and evolution of the promoter.
Biomedical Sciences

Biomedical Sciences

Journal of Bioanalysis & Biomedicine

Author(s): Buitkamp J, Jann O, Fries R

Abstract Share this page

Abstract Interleukin (IL)-13 plays a key role in the T-helper 2 type immune response. In domestic animal species no sequence information is available for the IL13 gene. Here, we report the isolation and sequencing of the cattle IL13 gene. Since a heterologous cDNA probe failed to hybridize to cattle genomic DNA the gene was identified by a positional sequencing approach. In human and mouse both cytokines are located within a region of about 30 kilobases (kb). Therefore the region upstream of the cattle IL4 gene has been sequenced by a shotgun approach. The cattle IL13 gene was found to be located about 20 kb upstream of IL4 in a head-to-tail orientation. The exon-intron structure and the promoter region is well conserved across species. The alignment of the IL13 sequences from different species allowed the identification of highly conserved amino acid positions and transcriptional elements. Two conserved amino acid positions (glu(31) and pro(94)) are most probably homologous to the interleukin-4 signalling residues. One of the potential binding sites for transcription factors is located at position -197 to -189 and is closely related to the P elements of the IL4 promoter. Nevertheless, the overall identity of the promoter sequences of IL13 and IL4 is low. Therefore we conclude that both cytokines use diverse regulatory elements. There is evidence that IL-13, but not IL-14, is the key mediator in allergic asthma. Furthermore IL-13 is involved in resistance to some infections. Therefore IL-13 is of interest for disease association studies in domestic animals.
This article was published in Immunogenetics and referenced in Journal of Bioanalysis & Biomedicine

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords