alexa The chaperonin ATPase cycle: mechanism of allosteric switching and movements of substrate-binding domains in GroEL.
Chemistry

Chemistry

Journal of Physical Chemistry & Biophysics

Author(s): Roseman AM, Chen S, White H, Braig K, Saibil HR

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Abstract Chaperonin-assisted protein folding proceeds through cycles of ATP binding and hydrolysis by the large chaperonin GroEL, which undergoes major allosteric rearrangements. Interaction between the two back-to-back seven-membered rings of GroEL plays an important role in regulating binding and release of folding substrates and of the small chaperonin GroES. Using cryo-electron microscopy, we have obtained three-dimensional reconstructions to 30 A resolution for GroEL and GroEL-GroES complexes in the presence of ADP, ATP, and the nonhydrolyzable ATP analog, AMP-PNP. Nucleotide binding to the equatorial domains of GroEL causes large rotations of the apical domains, containing the GroES and substrate protein-binding sites. We propose a mechanism for allosteric switching and describe conformational changes that may be involved in critical steps of folding for substrates encapsulated by GroES.
This article was published in Cell and referenced in Journal of Physical Chemistry & Biophysics

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