alexa The coming of age of liquid chromatography coupled to tandem mass spectrometry in the endocrinology laboratory.
Chemical Engineering

Chemical Engineering

Journal of Chromatography & Separation Techniques

Author(s): Carvalho VM

Abstract Share this page

Abstract Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been rapidly incorporated in the routine of the endocrinology laboratory. Most endocrinologists are aware of the benefits afforded by this technique and tandem mass spectrometers are clearly no longer a mere research method but an important tool widely used for diagnosis. In the last 15 years, LC-MS/MS has replaced techniques such as immunoassay and HPLC for the analysis of hormones because it provides higher specificity and good sensitivity. Also, it permits simultaneous measurement of several analytes and sample preparation and acquisition are fast and simple. Although several strategies based on LC-MS/MS have been described in the last 15 years, there is still room for improvement. The impact of matrix effects and isobaric interferences have been addressed by only a few studies, and standardization with reference materials is available for a limited number of analytes. This review summarizes the application of LC-MS/MS in analyzing three classes of hormones: steroids, derivatives of the aromatic amino acids, and peptides and proteins. The benefits and current limitations of LC-MS/MS will be discussed for these hormone categories. Copyright © 2011 Elsevier B.V. All rights reserved. This article was published in J Chromatogr B Analyt Technol Biomed Life Sci and referenced in Journal of Chromatography & Separation Techniques

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version