alexa The dynamics of vitellogenin gene expression differs between intact and eyestalk ablated kuruma prawn Penaeus (Marsupenaeus) japonicus
Geology & Earth Science

Geology & Earth Science

Journal of Oceanography and Marine Research

Author(s): Naoaki TSUTSUI, Yi Kyung KIM, Safiah JASMANI, Tsuyoshi OHIRA, Marcy N WILDER

Abstract Share this page

In order to compare the dynamics of vitellogenin gene expression between naturally maturing prawns and prawns induced to mature artificially by eyestalk ablation, a cDNA encoding vitellogenin was cloned from a cDNA library prepared from the hepatopancreas of the kuruma prawn Penaeus (Marsupenaeus) japonicus, and a quantitative real-time reverse transcription – polymerase chain reaction (RT-PCR) system was developed. Sequence analysis revealed the likely possibility that vitellogenin cDNA from the hepatopancreas was identical to that from the ovary which had been isolated in a previous study. Based on this information, a quantitative real-time RT-PCR system was established and the dynamics of vitellogenin mRNA levels were examined. In naturally maturing prawns, vitellogenin mRNA levels were maintained at low levels during the previtellogenic stage, and thereafter, levels increased as vitellogenesis progressed but decreased during the latter stages of maturation in the hepatopancreas and ovary. In contrast, in eyestalk-ablated prawns, changes in mRNA levels differed in both tissues; an obvious increment of mRNA levels was revealed in the ovary, whereas mRNA levels were negligible in the hepatopancreas. This suggests that eyestalk ablation cannot be used to accurately simulate the natural process of vitellogenin gene expression during vitellogenesis, and that vitellogenin gene expression is regulated in a tissue-specific manner.

This article was published in Fisheries Science and referenced in Journal of Oceanography and Marine Research

Relevant Expert PPTs

Relevant Speaker PPTs

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engine[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords