alexa The effect of chilling on membrane lipid phase transition in human oocytes and zygotes.
Medicine

Medicine

Anatomy & Physiology: Current Research

Author(s): Ghetler Y, Yavin S, Shalgi R, Arav A

Abstract Share this page

Abstract BACKGROUND: Chilling injury occurs when the cell membrane undergoes a transition from the liquid state to the gel state. Human oocytes and single-cell zygotes are of similar shape and size but the post-thawing survival rate of oocytes is poorer. We set out to investigate the possible difference in membrane lipid phase transition (LPT) temperature between the two cell types. METHODS: The LPT temperature was measured with a Fourier Transform Infrared analyser, which detects the change in the vibration frequency of the CH2 bond stretches of the membrane lipid molecules during temperature change. The LPT temperatures of unfertilized human oocytes, in vitro-matured oocytes, and immature germinal vesicle (GV) stage oocytes were compared with that of abnormally fertilized human zygotes. RESULTS: The LPT temperatures of zygotes and of mature and immature GV oocytes differ significantly from each other (10.0 +/- 1.2, 16.9 +/- 0.9 and 24.4 +/- 1.6 degrees C respectively; P < 0.05). CONCLUSIONS: Zygotes show a higher resistance to chilling injury compared to oocytes at different developmental stages; this might explain the relatively poor survival rates of cryopreserved human oocytes and indicates the necessity to adjust the cryopreservation protocols in order to minimize cryoinjury. This article was published in Hum Reprod and referenced in Anatomy & Physiology: Current Research

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords