Author(s): Clegg MS, Vertucci FJ, Walker C, Belanger M, Britto LR
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Abstract This study assessed the effectiveness of different concentrations of sodium hypochlorite (NaOCl), 2\% chlorhexidine (CHX) (Vista Dental Products, Racine, WI), and BioPure MTAD (Dentsply Endodontics-Tulsa Dental, Tulsa, OK). Intracanal contents were collected from 10 patients diagnosed with chronic apical periodontitis. The samples were cultured on hemisections of root apices to generate a polymicrobial biofilm. Each biofilm was separately immersed in 6\% NaOCl, 3\% NaOCl, 1\% NaOCl, 2\% CHX, 1\% NaOCl followed by BioPure MTAD, and sterile phosphate buffered solution (PBS). SEM analysis showed 6\% NaOCl and 3\% NaOCl were capable of disrupting and removing the biofilm; 1\% NaOCl and 1\% NaOCl followed by MTAD were capable of disrupting the biofilm, but not eliminating bacteria; 2\% CHX was not capable of disrupting the biofilm. Viable bacteria could not be cultured from specimens exposed to 6\% NaOCl, 2 \% CHX, or 1\% NaOCl followed by BioPure MTAD. These results indicate that 6\% NaOCl was the only irrigant capable of both rendering bacteria nonviable and physically removing the biofilm.
This article was published in J Endod
and referenced in Transplant Reports : Open Access