Author(s): Olgun EJ, Galicia S, AnguloGuerrero O, Hernndez E
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Abstract Evaluation of the effect of low light flux and nitrogen deficiency on growth and chemical composition of Spirulina sp. (straight filaments strain, SF) in batch cultures utilizing a complex medium containing sea-water supplemented with anaerobic effluents from digested pig waste, was undertaken. Cultivation was carried out either at a light flux of 66 (lower) or 144 micromol photon m(-2) s(-1) (higher), utilizing bench raceways. Biomass concentration (as dry weight) after 12 days of cultivation in the complex medium was similar (P < 0.05) to the one observed in a chemically defined medium (Zarrouk), regardless of the light intensity. Protein content of the biomass in the complex medium was significantly lower (P < 0.05), compared to the Zarrouk medium, regardless of the light flux. However, biomass from the complex medium was enriched in total lipids (28.6\%), when cultures were exposed to the lower light flux. On the other hand, the palmitoleic acid percentage of total fatty acids was significantly higher (P < 0.05) at a higher light intensity and a high level of gamma linolenic acid (GLA) as a percentage of total fatty acids was observed (28.13\%) in the biomass harvested from the complex medium at the lower light intensity. Finally, polysaccharide content was significantly higher (P < 0.05) at the high light intensity and a very high content of total polysaccharides (28.41\%) was observed in the complex medium.
This article was published in Bioresour Technol
and referenced in Journal of Microbial & Biochemical Technology