Author(s): M ZASLAN, T AYTEKIN, IH KILI, AI BOZKURT, E GLDR
We studied the effects of vitamin C supplementation on the leucocyte counts and exercise performance of mice. Mice were divided into 4 groups; 1 control and 3 experimental. We supplemented the diet of the mice from each experimental group with different doses of ascorbic acid (4 mg, 8.8 mg, and 13 mg/day; C4, C9, C13, respectively) by intra-peritoneal injection. Physiological serum was given to the control group (CON) via the same procedure. Exercise performance was based on swim time to fatigue. Blood samples were taken and evaluated at day 7, 14, 21, and 28. At the end of day 28, tissue samples were taken from different organs for pathological examination. The lymphocyte percentage was 40.2 ± 6 % for CON on day 28. For all C groups, the range of the lymphocyte percentage was 47.5 %- 57.1 % (p < 0.001). Swim time was 1.6 ± 0.3 min at day 28 for CON. This value was increased to 4.6- 8.8 min for the C groups (p < 0.001). No pathological differences in organs (skeleton muscle, stomach, spleen, kidney, liver, heart, skin, brain) were determined between the control and experimental groups. In conclusion, it was found that vitamin C supplementation increased the lymphocyte level in blood and improved swim time to fatigue.