alexa The First Draft of the Interaction Network of Endostatin, an Inhibitor of Angiogenesis
Oncology

Oncology

Journal of Oncology Research and Treatment

Author(s): RicardBlum S

Abstract Share this page

Endostatin is a C-terminal proteolytic fragment of collagen XVIII that is localized in vascular basement membrane zones in various organs, and inhibits angiogenesis and tumor growth. We have used protein and glycosaminoglycan arrays probed by surface plasmon resonance (SPR) to identify partners of endostatin, and to give further insights into its molecular mechanism of action. Glycosaminoglycans, matricellular proteins, collagens, the Abeta amyloid peptide, and transglutaminase-2 were found to bind endostatin. Endostatin was also shown to interact with intact pathogens (parasites invading the extracellular matrix) injected in buffer flow over SPR arrays, and could thus participate in host-pathogen interactions. The interaction network of endostatin was built using those experimental data and data available in the extracellular interaction database MatrixDB (http://matrixdb.ibcp.fr). The network was visualized with the software environment Cytoscape, and was annotated using UniProtKB, Gene Ontology and InterPro data. The predominant function associated with the endostatin network was cell adhesion. The most represented domains in the network were EGF (Epidermal Growth Factor) and EGF-like domains. 46% of endostatin partners bind calcium. Kinetics and affinity constants calculated by SPR experiments were integrated into the network to prioritize interactions according to their rate of formation and their stability. Data on binding sites were also integrated to discriminate simultaneous from mutually exclusive interactions. The integrated network was used as a framework to build a mathematical model of endostatin mechanism of action. We focus in the network established by endostatin at the cell surface, where it is able to bind to several receptors, to understand how endostatin selects a receptor and to determine if its binding to a receptor modify its interactions with other cell-surface associated molecules. The building of a dynamic interaction network will be helpful to understand how information/signaling is conveyed through the network and to predict the consequences of perturbations.

  • To read the full article Visit
  • Open Access
This article was published in J Biomol Tech. and referenced in Journal of Oncology Research and Treatment

Relevant Expert PPTs

Relevant Speaker PPTs

  • Werner Boecker
    Syringomatous tumour of the nipple and low-grade adenosquamous carcinoma: Evidence for a common origin
    PPT Version | PDF Version
  • Tibor Tot
    Multiparameter characterization of breast carcinoma: subgross, microscopy, proteins, and genes
    PPT Version | PDF Version
  • Fathia El Sharkawi
    The effect of PTEN and TRAIL genes loaded on nanoparticles on hepatocellular carcinoma
    PPT Version | PDF Version
  • Yosef Yarden
    Classically, the 3’untranslated region (3’UTR) is that region in eukaryotic protein-coding genes from the translation termination codon to the polyA signal. It is transcribed as an integral part of the mRNA encoded by the gene. However, there exists another kind of RNA, which consists of the 3’UTR alone, without all other elements in mRNA such as 5’UTR and coding region. The importance of independent 3’UTR RNA (referred as I3’UTR) was prompted by results of artificially introducing such RNA species into malignant mammalian cells. Since 1991, we found that the middle part of the 3’UTR of the human nuclear factor for interleukin-6 (NF-IL6) or C/EBP gene exerted tumor suppression effect in vivo. Our subsequent studies showed that transfection of C/EBP 3’UTR led to down-regulation of several genes favorable for malignancy and to up-regulation of some genes favorable for phenotypic reversion. Also, it was shown that the sequences near the termini of the C/EBP 3’UTR were important for its tumor suppression activity. Then, the C/EBP 3’UTR was found to directly inhibit the phosphorylation activity of protein kinase CPKC in SMMC-7721, a hepatocarcinoma cell line. Recently, an AU-rich region in the C/EBP 3’UTR was found also to be responsible for its tumor suppression. Recently we have also found evidence that the independent C/EBP 3’UTR RNA is actually exists in human tissues, such as fetal liver and heart, pregnant uterus, senescent fibroblasts etc. Through 1990’s to 2000’s, world scientists found several 3’UTR RNAs that functioned as artificial independent RNAs in cancer cells and resulted in tumor suppression. Interestingly, majority of genes for these RNAs have promoter-like structures in their 3’UTR regions, although the existence of their transcribed products as independent 3’UTR RNAs is still to be confirmed. Our studies indicate that the independent 3’UTR RNA is a novel non-coding RNA species whose function should be the regulation not of the expression of their original mRNA, but of some essential life activities of the cell as a whole.
    PPT Version | PDF Version
  • Devathri Nanayakkara
    Context specific role of deubiquitylase enzyme, USP9X in oral squamous cell carcinoma
    PPT Version | PDF Version
  • Giselle L Gotamco
    A case of hybrid carcinoma of the nose
    PDF Version
  • Igor Malyshev
    GENETIC FEATURES OF NO GENERATING SYSTEMS AND RESISTANT TO EHRLICH ASCITES CARCINOMA
    PPT Version | PDF Version
  • Lubna Mushtaque Vohra
    Metaplastic carcinoma of the breast and p16 positivity: What does it mean?
    PPT Version | PDF Version
  • Rubens Mendes Canuto de Oliveira
    Progressive Form Of Biliary And Hepatic Paracoccidioidomycosis, Simulating Cholangiocarcinoma
    PPT Version | PDF Version
  • Myron R Szewczuk
    Transcriptional factor Snail and MMP-9 signaling axis controls tumor neovascularization, growth and metastasis in mouse model of human ovarian carcinoma
    PPT Version | PDF Version
  • Mingsong Wu
    Identification of differentially expressed genes in human lung adenocarcinoma: ERGIC3 as a novel lung cancer-related gene
    PPT Version | PDF Version
  • Flavia Secco Tavares de Souza
    A Comparative Study Among Elective Conventional Surgery, Urgency/Emergency Conventional Approaching and Elective Videolaparoscopic Surgery on the Treatment of Hospitalized Patients at First Surgeric Clinic of Federal Hospital of Bonsucesso with a Diagnostic of Colorectal adenocarcinoma, between January 2010 and December 2012
    PPT Version | PDF Version
  • Ahmed G. Hegazi
    Influence of honey in mice bearing Ehrlich carcinoma on immune status
    PPT Version | PDF Version
  • Jian Jun Wei
    MicroRNAs and the target genes in early tumorigenesis of fallopian/ovarian carcinoma
    PPT Version | PDF Version
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords