alexa The first step of experimental study on hybrid trachea: use of cultured fibroblasts with artificial matrix.
Biomedical Sciences

Biomedical Sciences

Journal of Bioengineering & Biomedical Science

Author(s): Osada H, Takeuchi S, Kojima K, Yamate N

Abstract Share this page

Abstract Anastomotic stenosis secondary to developing granulation tissue prevents an artificial trachea of non-porous type model from a safe practical use. Taira reported that a non-porous artificial trachea having soft ends for anastomosis works better in this regards apparently by cushioning mechanical stimulations repeatedly applied to the anastomotic rings than most models ever studied have done. We hypothesized that hybridization of soft anastomotic ends of nonporous artificial trachea may further prevent granulation tissue at the anastomoses hopefully by leading epithelial growth earlier onto the inner surface of graft's ends. The graft of our use was made of a 5-cm-long knitted dacron tube with inner coating with silicone rubber covering the entire length but for a 5 mm segment at each end. To date six mongrel dogs have been subjected to test this hypothesis. A piece of subcutaneous tissue was obtained from each dog and prepared for culture of fibroblasts in MEM. These fibroblasts were then suspended in 0.1\% collagen with MEM in a container, in which a graft was placed for further culture. Following some 3 weeks of this preparation each graft was implanted to replace a 10-cartilage-ring-long defect of the mediastinal trachea of each donor dog. One dog is enjoying active life 11 months postop, and another 3 months. Four dogs died from anastomotic stenosis 2 to 3 months. Being encouraged by the fact that the anastomotic rings of the long term survivors show bronchoscopically patency more than 40\% of cut surface area, and some portion with possible epithelialization without granulation, we will report our further results, along with histological study.
This article was published in J Cardiovasc Surg (Torino) and referenced in Journal of Bioengineering & Biomedical Science

Relevant Expert PPTs

Relevant Speaker PPTs

Recommended Conferences

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords